关键词: 微血管构筑, 经脉线, 免疫组织化学, 三维重建, 猪

Abstract: Objective To make threedimensional picture reconstruction of microvasculars in bladder meridian of hindlimb area by using serial skin tissue sections of bladder meridian of hindlimb in mini-pig and the tool software. Methods Three-month-old mini-pig of China Agricultural University Ⅲ series, positioning the bladder meriadian. To obtain the skin tissue sanples with the volume of 1.2cm×0.5cm×0.5cm between the third lumbar and sacrum, which was marked with meridian line in the center. Then cutting a “V”type groove as alignment mark at the juncture of dermis reticular layer and subcutaneous tissue under the meridian line of sample (1mm distance to epidermis) by using scalpel. Vertical-sectional serial sections of skin (10μm thick) were made, 500 tussise sections were collected from every other piece of tussise sections, then the microvasculars were stained by the means of immunohistochemistry. Two-dimensional pictures were collected with microscrope by the direction of positioning mark, then three-dimensional pictures were constructed. Results The constructed microvasculars in bladder meridian of hindlimb area were tridimensional observed with naked eye. The three dimensional picture could be splitted optional and showed the construction of microvasculars in different directions. Conclusion The microvasculars in bladder meridian of hindlimb area can be reconstructed and observed from any angle by using the method of serial skin tissue sections and microvascular staining by immunohistoc

Key words: Microvascular construction, Meridian line, Immunohistochemistry, Threedimensional reconstruction, Pig